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mouse myod1 gene  (Addgene inc)


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    Structured Review

    Addgene inc mouse myod1 gene
    Induction of myogenic differentiation by <t>MyoD-VSV</t> (A) RNA genome structure of MyoD-VSV. (B) Schedule of myogenic differentiation of mESCs infected with MyoD-VSV. (C and D) Images and relative mRNA levels of mESCs infected with MyoD-VSV on days −1, 1, 3, 5, and 7. The y axis values indicate relative RNA levels normalized against GAPDH (RQ). Each data point indicates the mean of technical duplicates or triplicates. Error bars indicate the maximum and minimum RQ. Another set of repeated experiments is shown in <xref ref-type=Figures S5 A and S5B. (E) Immunofluorescence staining of differentiation-induced cells with or without guanine on day 7. The experiment was carried out independently from the experiment of (C and D). Blue, Hoechst green, MHC or VSV-N. Images from two additional experiments are shown in Figures S5 C and S5D. Scale bars, 100 μm. " width="250" height="auto" />
    Mouse Myod1 Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse myod1 gene/product/Addgene inc
    Average 93 stars, based on 2 article reviews
    mouse myod1 gene - by Bioz Stars, 2026-04
    93/100 stars

    Images

    1) Product Images from "Scalable control of stem cell fate by riboswitch-regulated RNA viral vector without genomic integration"

    Article Title: Scalable control of stem cell fate by riboswitch-regulated RNA viral vector without genomic integration

    Journal: Molecular Therapy

    doi: 10.1016/j.ymthe.2025.01.005

    Induction of myogenic differentiation by MyoD-VSV (A) RNA genome structure of MyoD-VSV. (B) Schedule of myogenic differentiation of mESCs infected with MyoD-VSV. (C and D) Images and relative mRNA levels of mESCs infected with MyoD-VSV on days −1, 1, 3, 5, and 7. The y axis values indicate relative RNA levels normalized against GAPDH (RQ). Each data point indicates the mean of technical duplicates or triplicates. Error bars indicate the maximum and minimum RQ. Another set of repeated experiments is shown in <xref ref-type=Figures S5 A and S5B. (E) Immunofluorescence staining of differentiation-induced cells with or without guanine on day 7. The experiment was carried out independently from the experiment of (C and D). Blue, Hoechst green, MHC or VSV-N. Images from two additional experiments are shown in Figures S5 C and S5D. Scale bars, 100 μm. " title="Induction of myogenic differentiation by MyoD-VSV (A) RNA genome structure of MyoD-VSV. (B) Schedule ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Induction of myogenic differentiation by MyoD-VSV (A) RNA genome structure of MyoD-VSV. (B) Schedule of myogenic differentiation of mESCs infected with MyoD-VSV. (C and D) Images and relative mRNA levels of mESCs infected with MyoD-VSV on days −1, 1, 3, 5, and 7. The y axis values indicate relative RNA levels normalized against GAPDH (RQ). Each data point indicates the mean of technical duplicates or triplicates. Error bars indicate the maximum and minimum RQ. Another set of repeated experiments is shown in Figures S5 A and S5B. (E) Immunofluorescence staining of differentiation-induced cells with or without guanine on day 7. The experiment was carried out independently from the experiment of (C and D). Blue, Hoechst green, MHC or VSV-N. Images from two additional experiments are shown in Figures S5 C and S5D. Scale bars, 100 μm.

    Techniques Used: Infection, Immunofluorescence, Staining



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    Addgene inc mouse myod1 gene
    Induction of myogenic differentiation by <t>MyoD-VSV</t> (A) RNA genome structure of MyoD-VSV. (B) Schedule of myogenic differentiation of mESCs infected with MyoD-VSV. (C and D) Images and relative mRNA levels of mESCs infected with MyoD-VSV on days −1, 1, 3, 5, and 7. The y axis values indicate relative RNA levels normalized against GAPDH (RQ). Each data point indicates the mean of technical duplicates or triplicates. Error bars indicate the maximum and minimum RQ. Another set of repeated experiments is shown in <xref ref-type=Figures S5 A and S5B. (E) Immunofluorescence staining of differentiation-induced cells with or without guanine on day 7. The experiment was carried out independently from the experiment of (C and D). Blue, Hoechst green, MHC or VSV-N. Images from two additional experiments are shown in Figures S5 C and S5D. Scale bars, 100 μm. " width="250" height="auto" />
    Mouse Myod1 Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse myod1 gene/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    mouse myod1 gene - by Bioz Stars, 2026-04
    93/100 stars
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    Induction of myogenic differentiation by MyoD-VSV (A) RNA genome structure of MyoD-VSV. (B) Schedule of myogenic differentiation of mESCs infected with MyoD-VSV. (C and D) Images and relative mRNA levels of mESCs infected with MyoD-VSV on days −1, 1, 3, 5, and 7. The y axis values indicate relative RNA levels normalized against GAPDH (RQ). Each data point indicates the mean of technical duplicates or triplicates. Error bars indicate the maximum and minimum RQ. Another set of repeated experiments is shown in <xref ref-type=Figures S5 A and S5B. (E) Immunofluorescence staining of differentiation-induced cells with or without guanine on day 7. The experiment was carried out independently from the experiment of (C and D). Blue, Hoechst green, MHC or VSV-N. Images from two additional experiments are shown in Figures S5 C and S5D. Scale bars, 100 μm. " width="100%" height="100%">

    Journal: Molecular Therapy

    Article Title: Scalable control of stem cell fate by riboswitch-regulated RNA viral vector without genomic integration

    doi: 10.1016/j.ymthe.2025.01.005

    Figure Lengend Snippet: Induction of myogenic differentiation by MyoD-VSV (A) RNA genome structure of MyoD-VSV. (B) Schedule of myogenic differentiation of mESCs infected with MyoD-VSV. (C and D) Images and relative mRNA levels of mESCs infected with MyoD-VSV on days −1, 1, 3, 5, and 7. The y axis values indicate relative RNA levels normalized against GAPDH (RQ). Each data point indicates the mean of technical duplicates or triplicates. Error bars indicate the maximum and minimum RQ. Another set of repeated experiments is shown in Figures S5 A and S5B. (E) Immunofluorescence staining of differentiation-induced cells with or without guanine on day 7. The experiment was carried out independently from the experiment of (C and D). Blue, Hoechst green, MHC or VSV-N. Images from two additional experiments are shown in Figures S5 C and S5D. Scale bars, 100 μm.

    Article Snippet: The mouse Nanog sequence was cloned from the total RNA extracted from BRC6 mESCs, and the mouse Myod1 gene was obtained from LV-TRE-WT mouse MyoD-T2A-dsRedExpress2 (Addgene #60624) by PCR.

    Techniques: Infection, Immunofluorescence, Staining